Callery Pear, anthers, Heesch, Netherlands
This is a 5:1 macro, a short stack to only bring out detail specifically to the anthers of this 2cm flower.
Creative-wise, I like the result, as once you can do deep stacks automatically, there's a tendency to always use it. This shows that you don't always need to. A short stack can be interesting as well.
In terms of sharpness, I find it acceptable. I would want the anthers to be a bit sharper, to show a little more detail. Yet at 5x macro, this is no easy task, everything has to be perfect.
"Pyrus calleryana", or the Callery pear, is a species of pear tree native to China and Vietnam, in the family Rosaceae. It is most commonly known for its cultivar 'Bradford' and its offensive odor, widely planted throughout the United States and increasingly regarded as an invasive species.
comments (10)
New gear for the 5:1? Posted 5 years ago
https://www.wemacro.com/
...which sets in motion other small purchases like specimen holders, mini tripods, lights. Posted 5 years ago
That said, I know nothing about steppers. Whilst everybody's budget is different, I'm personally impressed by how affordable it is. This thing is rock solid and does exactly what it says. It's not some cheap low-end product. It easily competes with rails costing 1500$ or more. Posted 5 years ago
A few years back I helped a little (very little really) when a friend of mine created a setup to take series of stacks of chicken embryos with growth defects from 45 degrees above and below while rotating the embryo and later "stitching" or rather "converting" the stacks into a 3D model of each embryo for later reference so you could rotate the embryo on screen to look at it from all sides. The setup worked and most of it quite automagically, but it was a genuine PITA to get the lighting even enough to avoid "shade" artefacts in the 3D.
Just saying: It _should_ be possible to automate much of the process, but before you have that all figured out ... yeah ... time .... Posted 5 years ago
I had never seen a 3D stack until about 2 weeks ago. It was not a single dimension rotation, instead a "fly-by" around some crazy fungus at very high magnification. It was built from 19,000 images.
Which had me thinking....do this 20 times and your shutter/sensor is dead.
The artifacts indeed seem to be the biggest pain point. The way I see it, the stacking software is lacking essential information. It's basically just guessing based on an interpretation of pixel values. It's reverse engineering the 3d subject from flat pixels. If it had more depth information, it should be able to perform much better. But I guess that's a pretty simplistic thought. Posted 5 years ago
The PC (Core i7) had a hard time crunching though ... ran most of one night (4-6 hrs?) for one embryo I think it was ;o) Posted 5 years ago, modified 5 years ago
Not trying to convince you, just saying its excellent value for money. A steal, really. Posted 5 years ago