Promoted
Leccinellum quercophilum
Notes:
Habitat/Location: Top of forest ridge, growing in thick mulch/leaves. In a dense mixed hardwood forest in Northwest Georgia.
Pores: Golden brown. Tube layer is quite deep (almost 2 cm towards center). The pores themselves don’t seem to bruise to blue, but the flesh bruises quickly.
Stipe: cream to white, slightly scaly. dark brown ring near top.
Cap: Large (around 10cm diameter). Light brown to gold with striking blue to violet bruising.
Spore print color: Hard to acquire, dark ash brown
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Sequenced by Igor Safanov/Dr. Kudzma:
I sent a sample of this beauty off for sequencing back in 2017, and this was Igor's reply to me this week:
"finally got your bolete sequenced.
Dr. Kudzma obtained a nice full-length ITS sequence. It’s very similar (98.6% similar) to the ITS of Leccinellum quercophilum holotype. There are a number of differences, but more than half of them are ambiguous bases, not real mismatches, so the real similarity is over 99%. I asked Dr. Kudzma to proofread the sequence one more time to make sure there are no lingering errors.
There is nothing else so close in GenBank, so I am pretty certain your collection is L. quercophylum. Interestingly, this species thus far is known to be distributed in the northern tier of the country (east of the Mississippi River), so your collection is a notable geographical outlier."
A species of mushroom in the Boletaceae family.
comments (3)
The full-length nrITS sequence of MO295969 is 785 bps long (ITS1 = 146 bps, 5.8S = 161 bps, and ITS2 = 478 bps). There is a single ambiguity, an “R” (A/G) at position #114. Following my proofreading request, Dr. Kudzma confirmed no changes to the original sequence.
As per the comment below, overall this sequence is a very good match to the ITS derived from the holotype of L. quercophilum (KC691207). The latter sequences has 6 ambiguities – all are an “N” (any base). The two sequences differ at 11 positions:
> KC691207 has two gaps in the mixed homopolymeric motif (bases #72-79) early in ITS1: CCC-A-TT vs. CCCCATTT in MO295969.
> N vs. T at #103
> N vs. R. at #114
> N vs. C at #258
> N vs. T at #319
> In the ITS2 region, bases #473-482 in MO295969 are AAATAAAATT, whereas KC691207 has a gap at #476, a “C” at #478, and an “N” at #482.
7) C vs. T at #594
8) N vs. A at #638
Of these 11 mismatches only three are real/significant: #476, #478, and #594. Posted 6 years ago